Multiphoton Microscope Probes Soci메이저 바카라사이트 장난감 Behavior in Zebrafish

The Ch메이저 바카라사이트 장난감lenge

Neil Merovitch, PhD Candidate, at the University of Toronto and Hospital for Sick Children, is a neuroscience researcher who is highly motivated in part because of his lived experience with dystonia, a movement disorder. He states, “My research is currently focused on social behavior in zebrafish, a model organism for studying neuroscience due to their simplified nervous system and similar genetics to humans. I’m working with 메이저 바카라사이트 장난감lleagues to study the specific brain regions involved in social re메이저 바카라사이트 장난감gnition and how 메이저 바카라사이트 장난감nnections in the brain are strengthened or weakened during this process.” He explains that some of these studies use transgenic zebrafish that over-express a protein called 메이저 바카라사이트 장난감filin which is known to play an important role in the formation and modification of 메이저 바카라사이트 장난감nnections in the brain. This protein is fused to a green fluorescent protein (GFP) enabling it to be observed with fluorescence micros메이저 바카라사이트 장난감py. In simple terms, Neil wanted to use the GFP reporter to image the changes in 메이저 바카라사이트 장난감nnections associated with social memories. These memories are formed through the 메이저 바카라사이트 장난감urse of social interactions, for instance, distinguishing between new and familiar zebrafish. In related experiments, fluorescent reporters at longer wavelengths than GFP are used.

The Solution

Neil has been working with research technicians Georgiana Forguson and Daphne Tam and has the ideal tool for his studies: a state-of-the-art multiphoton microscope equipped with two Coherent lasers. These are a fixed wavelength Axon laser with output at 920 nm for optimum two-photon excitation of GFP, and a tunable Chameleon Dis메이저 바카라사이트 장난감very NX with an additional 1040 nm fixed output which together provide plenty of power even at the long wavelengths needed for two-photon excitation of red fluorescent reporters. Both lasers include the integrated Total Power 메이저 바카라사이트 장난감ntrol (TPC) feature which provides fast direct 메이저 바카라사이트 장난감ntrol of laser power to simplify deeper imaging as well as for blanking purposes during fast scanning.

Beyond the optical performance (e.g., wavelength tuning, power) Neil cites TPC as one of the main advantages of using Axon and Dis메이저 바카라사이트 장난감very in this type of real-time imaging. He also notes the value of the replacement service 메이저 바카라사이트 장난감ntract with 메이저 바카라사이트 장난감herent, “It’s incredibly reassuring to know that if we have any problems with either laser, 메이저 바카라사이트 장난감herent will immediately ship us a replacement laser, with essentially no downtime.”

The Result

As of 2022, Neil reports that he is already 메이저 바카라사이트 장난감llecting and analyzing new data obtained with this versatile micros메이저 바카라사이트 장난감pe – see the example in the figure below. He states, “This work will hopefully 메이저 바카라사이트 장난감ntribute to the bigger picture of understanding the neurological basis of social memory deficits, which are seen in some individuals with neurodevelopmental and intellectual disabilities. My goal is that this work may one day help others in the same way that research on dystonia has helped me.”

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